Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar.
Identifieur interne : 000538 ( Main/Exploration ); précédent : 000537; suivant : 000539Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar.
Auteurs : Chunfen Fan [République populaire de Chine] ; Hua Yu [République populaire de Chine] ; Shifei Qin [République populaire de Chine] ; Yongli Li [République populaire de Chine] ; Aftab Alam [République populaire de Chine] ; Changzhen Xu [République populaire de Chine] ; Di Fan [République populaire de Chine] ; Qingwei Zhang [République populaire de Chine] ; Yanting Wang [République populaire de Chine] ; Wanbin Zhu [République populaire de Chine] ; Liangcai Peng [République populaire de Chine] ; Keming Luo [République populaire de Chine]Source :
- Biotechnology for biofuels [ 1754-6834 ] ; 2020.
Abstract
Background
As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plant-specific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed
Results
Compared with wild type, the
Conclusions
This study has demonstrated a powerful strategy to enhance cellulosic bioethanol production by regulating brassinosteroid biosynthesis for reducing lignocellulose recalcitrance in the transgenic poplar plants. It has also provided a green-like process for biomass pretreatment and enzymatic saccharification in poplar and beyond.
DOI: 10.1186/s13068-020-1652-z
PubMed: 31988661
PubMed Central: PMC6969456
Affiliations:
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sort="Zhang, Qingwei" uniqKey="Zhang Q" first="Qingwei" last="Zhang">Qingwei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yanting" sort="Wang, Yanting" uniqKey="Wang Y" first="Yanting" last="Wang">Yanting Wang</name><affiliation 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Biotechnology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Peng, Liangcai" sort="Peng, Liangcai" uniqKey="Peng L" first="Liangcai" last="Peng">Liangcai Peng</name><affiliation wicri:level="3"><nlm:affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 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green-like biomass process in transgenic poplar.</title><author><name sortKey="Fan, Chunfen" sort="Fan, Chunfen" uniqKey="Fan C" first="Chunfen" last="Fan">Chunfen Fan</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Yu, Hua" sort="Yu, Hua" uniqKey="Yu H" first="Hua" last="Yu">Hua Yu</name><affiliation wicri:level="3"><nlm:affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Qin, Shifei" sort="Qin, Shifei" uniqKey="Qin S" first="Shifei" last="Qin">Shifei Qin</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Li, Yongli" sort="Li, Yongli" uniqKey="Li Y" first="Yongli" last="Li">Yongli Li</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Alam, Aftab" sort="Alam, Aftab" uniqKey="Alam A" first="Aftab" last="Alam">Aftab Alam</name><affiliation wicri:level="3"><nlm:affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Xu, Changzhen" sort="Xu, Changzhen" uniqKey="Xu C" first="Changzhen" last="Xu">Changzhen Xu</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Fan, Di" sort="Fan, Di" uniqKey="Fan D" first="Di" last="Fan">Di Fan</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Qingwei" sort="Zhang, Qingwei" uniqKey="Zhang Q" first="Qingwei" last="Zhang">Qingwei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Yanting" sort="Wang, Yanting" uniqKey="Wang Y" first="Yanting" last="Wang">Yanting Wang</name><affiliation wicri:level="3"><nlm:affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Zhu, Wanbin" sort="Zhu, Wanbin" uniqKey="Zhu W" first="Wanbin" last="Zhu">Wanbin Zhu</name><affiliation wicri:level="3"><nlm:affiliation>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Peng, Liangcai" sort="Peng, Liangcai" uniqKey="Peng L" first="Liangcai" last="Peng">Liangcai Peng</name><affiliation wicri:level="3"><nlm:affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Luo, Keming" sort="Luo, Keming" uniqKey="Luo K" first="Keming" last="Luo">Keming Luo</name><affiliation wicri:level="1"><nlm:affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing</wicri:regionArea><wicri:noRegion>Chongqing</wicri:noRegion></affiliation></author></analytic><series><title level="j">Biotechnology for biofuels</title><idno type="ISSN">1754-6834</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>Background</b></p><p>As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plant-specific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed </p></div><div type="abstract" xml:lang="en"><p><b>Results</b></p><p>Compared with wild type, the </p></div><div type="abstract" xml:lang="en"><p><b>Conclusions</b></p><p>This study has demonstrated a powerful strategy to enhance cellulosic bioethanol production by regulating brassinosteroid biosynthesis for reducing lignocellulose recalcitrance in the transgenic poplar plants. It has also provided a green-like process for biomass pretreatment and enzymatic saccharification in poplar and beyond.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">31988661</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1754-6834</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Biotechnology for biofuels</Title><ISOAbbreviation>Biotechnol Biofuels</ISOAbbreviation></Journal><ArticleTitle>Brassinosteroid overproduction improves lignocellulose quantity and quality to maximize bioethanol yield under green-like biomass process in transgenic poplar.</ArticleTitle><Pagination><MedlinePgn>9</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13068-020-1652-z</ELocationID><Abstract><AbstractText Label="Background" NlmCategory="UNASSIGNED">As a leading biomass feedstock, poplar plants provide enormous lignocellulose resource convertible for biofuels and bio-chemicals. However, lignocellulose recalcitrance particularly in wood plants, basically causes a costly bioethanol production unacceptable for commercial marketing with potential secondary pollution to the environment. Therefore, it becomes important to reduce lignocellulose recalcitrance by genetic modification of plant cell walls, and meanwhile to establish advanced biomass process technology in woody plants. Brassinosteroids, plant-specific steroid hormones, are considered to participate in plant growth and development for biomass production, but little has been reported about brassinosteroids roles in plant cell wall assembly and modification. In this study, we generated transgenic poplar plant that overexpressed <i>DEETIOLATED2</i> gene for brassinosteroids overproduction. We then detected cell wall feature alteration and examined biomass enzymatic saccharification for bioethanol production under various chemical pretreatments.</AbstractText><AbstractText Label="Results" NlmCategory="UNASSIGNED">Compared with wild type, the <i>PtoDET2</i> overexpressed transgenic plants contained much higher brassinosteroids levels. The transgenic poplar also exhibited significantly enhanced plant growth rate and biomass yield by increasing xylem development and cell wall polymer deposition. Meanwhile, the transgenic plants showed significantly improved lignocellulose features such as reduced cellulose crystalline index and degree of polymerization values and decreased hemicellulose xylose/arabinose ratio for raised biomass porosity and accessibility, which led to integrated enhancement on biomass enzymatic saccharification and bioethanol yield under various chemical pretreatments. In contrast, the CRISPR/Cas9-generated mutation of <i>PtoDET2</i> showed significantly lower brassinosteroids level for reduced biomass saccharification and bioethanol yield, compared to the wild type. Notably, the optimal green-like pretreatment could even achieve the highest bioethanol yield by effective lignin extraction in the transgenic plant. Hence, this study proposed a mechanistic model elucidating how brassinosteroid regulates cell wall modification for reduced lignocellulose recalcitrance and increased biomass porosity and accessibility for high bioethanol production.</AbstractText><AbstractText Label="Conclusions" NlmCategory="UNASSIGNED">This study has demonstrated a powerful strategy to enhance cellulosic bioethanol production by regulating brassinosteroid biosynthesis for reducing lignocellulose recalcitrance in the transgenic poplar plants. It has also provided a green-like process for biomass pretreatment and enzymatic saccharification in poplar and beyond.</AbstractText><CopyrightInformation>© The Author(s) 2020.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Chunfen</ForeName><Initials>C</Initials><Identifier Source="ORCID">0000-0002-8739-3470</Identifier><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Hua</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</Affiliation><Identifier Source="ISNI">0000 0004 1790 4137</Identifier><Identifier Source="GRID">grid.35155.37</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Qin</LastName><ForeName>Shifei</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yongli</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Alam</LastName><ForeName>Aftab</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</Affiliation><Identifier Source="ISNI">0000 0004 1790 4137</Identifier><Identifier Source="GRID">grid.35155.37</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Changzhen</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Di</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qingwei</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yanting</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</Affiliation><Identifier Source="ISNI">0000 0004 1790 4137</Identifier><Identifier Source="GRID">grid.35155.37</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Wanbin</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.</Affiliation><Identifier Source="ISNI">0000 0004 0530 8290</Identifier><Identifier Source="GRID">grid.22935.3f</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Liangcai</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>2Biomass & Bioenergy Research Centre, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070 China.</Affiliation><Identifier Source="ISNI">0000 0004 1790 4137</Identifier><Identifier Source="GRID">grid.35155.37</Identifier></AffiliationInfo><AffiliationInfo><Affiliation>3College of Biomass Sciences and Engineering, College of Agronomy and Biotechnology, China Agricultural University, Beijing, 100193 China.</Affiliation><Identifier Source="ISNI">0000 0004 0530 8290</Identifier><Identifier Source="GRID">grid.22935.3f</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Luo</LastName><ForeName>Keming</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>1Chongqing Key Laboratory of Plant Resource Conservation and Germplasm Innovation, Key Laboratory of Eco-environments of Three Gorges Reservoir Region, Ministry of Education, Institute of Resources Botany, School of Life Sciences, Southwest University, Chongqing, 400715 China.</Affiliation><Identifier Source="GRID">grid.263906.8</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>01</Month><Day>18</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Biotechnol Biofuels</MedlineTA><NlmUniqueID>101316935</NlmUniqueID><ISSNLinking>1754-6834</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bioethanol</Keyword><Keyword MajorTopicYN="N">Brassinosteroid</Keyword><Keyword MajorTopicYN="N">Green-like pretreatment</Keyword><Keyword MajorTopicYN="N">Lignocellulose modification</Keyword><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">Saccharification</Keyword><Keyword MajorTopicYN="N">Xylem differentiation</Keyword></KeywordList><CoiStatement>Competing interestsThe authors declare that they have no competing interests.</CoiStatement></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>01</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>epublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31988661</ArticleId><ArticleId IdType="doi">10.1186/s13068-020-1652-z</ArticleId><ArticleId 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sort="Xu, Changzhen" uniqKey="Xu C" first="Changzhen" last="Xu">Changzhen Xu</name><name sortKey="Yu, Hua" sort="Yu, Hua" uniqKey="Yu H" first="Hua" last="Yu">Hua Yu</name><name sortKey="Zhang, Qingwei" sort="Zhang, Qingwei" uniqKey="Zhang Q" first="Qingwei" last="Zhang">Qingwei Zhang</name><name sortKey="Zhu, Wanbin" sort="Zhu, Wanbin" uniqKey="Zhu W" first="Wanbin" last="Zhu">Wanbin Zhu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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